Bolt wont be running in Rome, but De Grasse is looking forward to racing the Jamaican great at the world championships in London in August.
Bolt hasn't lost an individual race since 2013 and his streak appears to be safe with Saturday's scheduled 100 metres as he will race mainly against his Jamaican teammates.
"When he won the double sprint gold medals in Beijing 2008 and then added the sprint relay gold also in record time, it had an effect right across the spectrum in Jamaica because all the high schools reported that the number of athletes coming out to train to make their school teams for Boys and Girls Championship doubled".
Asafa Powell withdrew this week with an injury leaving Bolt with Nesta Carter, Michael Frater and Nickel Ashmeade in his heat.
Bolt was joined on the podium at the news conference by six other track stars, including British distance runner Mo Farah, six-time Olympic gold medal victor Allyson Felix and world and Olympic 400 metre champion Wayde van Niekerk.
"For me it will just be a joy", the world's fastest man told AFP on Thursday. I love Jamaica, I love competing here, it's always so much fun and I know it's going to be a special night paying tribute to Usain.
"The energy that's going to be there is just going to be brilliant and it's definitely going to be emotional for me because it's my last one and so many Jamaican fans will be there".
And his sudden death at the age of 34 knocked Bolt so badly that the eight-time Olympic gold medallist could not face training.
No Powerball victor last night, new jackpot estimated at $435M
At Kroger at Marketplace Square Drive, people have also been buying tickets in hopes that they may have the winning numbers . The jackpot for Saturday's Powerball drawing is an estimated $435 million, according to the Massachusetts State Lottery.
He ran 19.90 on April 22 and is the joint-second fastest over the 200m this year. "At the start it really took us by surprise and it kind of set me back a bit training-wise, because mentally I wasn't ready to even train for two weeks or two and a half weeks", he said.
For Bolt, after his experience at the 2012 Olympics in the British capital, the decision on where to hang up his spikes for good was a simple one.
Twelve years of hard work have brought Bolt the level of supremacy and excellence he has achieved, Glen Mills, his coach since 2005, told Reuters.
"I had to take off and just try to collect myself".
"We are here to honour Usain and what he done for the sport".
"For me there is some sadness unless he changes his mind", Mills said.
"I just wanted to come out here and put on a show for the crowd and thank them for the support".